Scientist II

Scientist II#25-66823

South San Francisco, CA

All On-site

Job Description

Manager is open to reviewing candidates at a Scientist I level

Scientist I:

1 to 3 years of experience

Max Bill Rate: ***

Scientist II:

3 to 5 years of experience

Max Bill Rate: ***

Position Title - Scientist I/II

Location/Site - South San Francisco (1000 Gateway blvd)

Cost Center - ...

Number of positions needed - 1

Job description (feel free to attach) - see attached document

Tentative start date - ASAP

Tentative end date - 1 year from start date

Work shift (days/times) - Mon - Friday / standard working hours (flexible)

Weekends included (Y/N) - N

Remote, 100% Onsite or Hybrid Onsite Schedule - 100 Onsite

Travel required (Y/N) - N

Is *** system access required for this worker (Y/N) - Y

What *** equipment is needed? - laptop

Is an *** email required (Y/N) - Y

Top 3-5 skills needed -

CHO cell culture

CHO cell line development

Molecular Biology (DNA/RNA extraction)

Possible temp to perm position - possible

Number of Interviews (recommend minimizing) - 2 round ; 1 VC and 1 onsite

Reason for request - Business/Project work needs hands-on support for critical CMC cell line development innovation projects.

The Cell Line Development Group in South San Francisco, CA is seeking a Scientist I/II to join a talented and collaborative team developing CHO cell lines for GMP applications. In this laboratory position the candidate will perform hands-on CHO cell line development and characterization studies in a well-supported and dynamic environment to help advance Client biotherapeutics into human clinical studies. The work will encompass molecular, cellular, and bioprocess development studies relating to the generation and characterization of optimized CHO cell lines for production of our cancer pipeline candidates.

Key Responsibilities:

Perform CHO cell line development, including transfection, selection, single cell subcloning, growth and productivity screening, cell banking and stability assessment of cell banks.

Design and generate DNA vector constructs for protein?including monoclonal antibodies and antibody-like molecules)

Culture mammalian cells across multiple scales and formats, from 96-well plates up to shake flasks.

Operate instruments like ViCell automated cell counter, Octet, Clone Select Imager, microscope, HPLC, ambr15, Beacon and flow cytometer. Share responsibility for equipment maintenance.

Perform genetic characterization of cell banks using molecular techniques like DNA sequencing and PCR.

Review DNA sequencing data (including NGS), Southern/Northern blots, quantitative PCR results.

Author and review cell line development technical reports that meet expectations of regulatory agencies for all stages from IND to BLA.

Present updates at cross-functional meetings and collaborate closely with cell culture, purification, analytical and formulation development colleagues to meet project timelines.

Actively seek opportunities to optimize or enhance the cell line development workflow.

May publish or present scientific findings in peer-reviewed journals, internal or external conferences or consortia.

Display effective organization, verbal and written communication skills, and adherence to good documentation practices.

Qualifications

Basic:

Scientist I: BS with a minimum of 5 years or MS with a minimum of 3 years of relevant experience

Scientist II: BS with a minimum of 7 years or MS with a minimum of 5 years of relevant experience

Proficient in aseptic techniques, mammalian cell culture and basic molecular biology techniques (molecular cloning, plasmid preps, DNA digests/gels, PCR/qPCR)

Experienced in developing stably transfected CHO cell lines for production of monoclonal antibodies and antibody-like molecules.

Strong understanding of mammalian cell biology and molecular biology concepts.

Must have excellent attention to detail and must keep detailed records in electronic lab notebook on a daily basis.

Demonstrated ability to independently design, execute and interpret critical experiments to answer scientific questions.

Demonstrated ability to learn, understand and master new experimental techniques.

Demonstrated ability to work collaboratively within and outside of the group.

Demonstrated ability to author technical reports and presentations.

Proficient with Microsoft Office software and able to learn new software applications.

Able to multi-task and work within timelines.

Strong communication skills - verbal, written, scientific data presentation.

Preferred:

Background in CHO cell line development for GMP applications within biotech/pharma industry.

Understanding of cell biology and bioprocess engineering concepts.

Hands-on experience running RNA-seq workflow and analysis

Experience in bioinformatics software to analyze DNA and RNA for sequence confirmation and expression analysis

Proficiency in programming/data science languages, such as R, Python, and Java

Experience with flow cytometry

Experience with ddPCR

Experience with ambr15 operation

EEO:

Mindlance is an Equal Opportunity Employer and does not discriminate in employment on the basis of - Minority/Gender/Disability/Religion/LGBTQI/Age/Veterans.