Scientist I

Scientist I#25-65043

Cambridge, MA

All On-site

Job Description

Immunology R D organization is seeking a highly motivated contractor to join our Systems Immunology early discovery team. We are looking for a candidate who is passionate about learning and has excellent attention to detail, to support target identification and validation efforts within our Immunology R D division. The ideal candidate will have academic or industry experience in molecular and cell biology, with a preference for expertise in single-cell technologies, including single-cell next-generation sequencing (NGS), flow cytometry, and human cell culture. A willingness to learn and develop new techniques, as well as engage in in-depth analyses using diverse approaches, is essential. This role involves collaboration with various teams across R D and other departments.

Key Responsibilities:

Conduct high-throughput functional experiments with immune cells in vitro and characterize outcomes at the single-cell level using various omics techniques.

Perform scRNAseq library preparations manually or with automated liquid handlers (e.g., Biomek i7) as part of a team.

Operate flow cytometry and sorting machines to characterize the single-cell proteome and isolate targeted cells.

Utilize Illumina sequencers and other laboratory instruments for NGS library quality control (e.g., Tapestation).

Accurately document project details, protocols, and experimental procedures in the Electronic Lab Notebook (ELN).

Conduct experiments and optimize protocols independently post-training, collaborating with colleagues to deliver high-quality data promptly.

Interpret findings and effectively communicate scientific data, concepts, and recommendations to internal audiences.

Collaborate with computational biologists and data scientists to generate and interpret scOMICs datasets.

Work in ***'s matrixed environment to support various departments, participate in alignment meetings, and facilitate cross-functional collaboration.

Required Qualifications:

BS or equivalent education with 1-3+ years of experience or MS or equivalent education (PhD overqualified)

Strong background in basic molecular biology and cellular biology/or genomics with experience in molecular biology lab techniques such as flow cytometru and DNA/RNA extraction, as well as familiarity with PBMC isolation, handling and cell culture.

Preferred Qualifications:

Experience with flow cytometry.

Experience with human cell culture and functional experiments.

Familiarity with 10X Genomics platforms.

Background in R and immunology is a plus but not required.

EEO:

Mindlance is an Equal Opportunity Employer and does not discriminate in employment on the basis of - Minority/Gender/Disability/Religion/LGBTQI/Age/Veterans.