Scientist - II

Scientist - II
Location: South San Francisco, CA
Duration: 12 Months

Department summary:
We are Discovery Biologics, and we are on a quest for cures and are committed to be the world's premier, most research-intensive biopharmaceutical company. We identify and target steps in disease mechanisms or pathways that could be inhibited or enhanced. Our goal is to isolate a compound that is effective against a disease target. Using innovative thinking, state-of-the-art facilities and robust scientific methodology we collaborate to Client the next medical breakthrough.
Our teams combine leading drug discovery capabilities and world-class R&D with the purpose of turning breakthrough science into life-changing medicines.

We are looking for a motivated scientist to be an integral part of the Protein Science department within Discovery Biologics at Client Research Labs (MRL)- South San Francisco.
• The successful candidate will be involved in multiple aspects of the production of recombinant proteins, monoclonal antibodies, multispecific scaffolds, and Client protein modalities to enable all aspects of Early Discovery.
• Ideally, the candidate will be able to execute and develop on techniques involving recombinant protein expression in CHO cells using both transient and stable-pool techniques.
• Additionally, the candidate should have foundational experience in purification and QC, along with a proven ability to accurately record and present data.

Job responsibilities in detail:
• Maintain and propagate CHO cells in suspension culture independently to support ongoing projects in a cell culture lab.
• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293). Express and batch purify a variety of proteins (monoclonal, bispecific antibodies, VHHs, Fabs, Fc-Fusions, etc.) using both resin and magnetic-bead immunoprecipitation methods.
• Execute fed-batch experiments in a variety of platforms: shake flasks 125ml to 5L, tube spins and multi-well plates.
• Perform analytical measurements for cell growth, metabolite measurements and HPLC analysis of titers from supernatant.
• Analyze purified proteins using techniques: SDS-PAGE, HPLC, UPLC, CESDS, Nano-drop, UV-Vis.
• Maintain and troubleshoot mammalian cell culture systems.
• Document protein production processes, prepare reports and communicate results
• ELN documentation.

Quals--
Education and experience required:
• B.S. degree in Biochemistry, Cell/Molecular Biology, Bioprocess engineering or related fields and three (3) years of industry or academia experience or a M.S. degree with 1 year of experience in similar fields

Must have:
• Prior experience in mammalian cell culture techniques, and cell culture media preparation is a prerequisite: single use mammalian cell culture tools employed in bioprocess development, including but not limited to deep well plates and shake flasks.
• Perform transient and stable transfections for protein expression in mammalian cells (CHO, HEK293).
• Ability to multi-task different protocols at once, handle concurrent projects simultaneously with high attention to detail.

Nice to have/preferred:
• Experience with high-throughput systems and automation.
• Experience with batch purification using a variety of scaffolds and basic QC.
• Industry experience is preferred.

Software skills:
• Basic knowledge on biological data collection and analysis tools (Vicell, metabolite analyzers) and computer applications such as ELN, MS Word, MS Excel and MS PowerPoint.
• Experience with data and reagent management software such as Dotmatics, Benchling, Spotfire, etc is preferred.

Skills/techniques needed:
• Excellent aseptic techniques and familiarity with principles of sterilization/disinfection.
• Strong communication, organizational, and interpersonal skills.
• Able to maintain a "can-do” attitude and act with a sense of urgency to complete innovative projects while being flexible in work priorities based on business needs.
• Understand and adhere to corporate standards regarding code of conduct, safety, appropriate handling of materials
• Able to troubleshoot cell culture system and think critically about experiments.
• Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work.

Note:
• Onsite role.
• 12 months assignment.
• Lab based role.
• Do not submit PHD.

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