Staff Scientist, Biochemistry, Schey Lab

About the Work Unit:

The mission of the Mass Spectrometry Research Center (MSRC) is to bring state-of-the-art mass spectrometry expertise, methodology, and instrumentation to researchers at Vanderbilt University and Vanderbilt University Medical Center. The MSRC is located on the 9th floor in Medical Research Building III. The MSRC Schey lab occupies 2,500 square feet of laboratory space, and has two LC-MS/MS instruments, including one LTQ Velos ion trap and a Waters Xevo G2S QTOF instrument with HDX capabilities. Support for the laboratory comes from NIH- and ARPA-H funded projects.

Key Functions and Expected Performance:

Research

• Execution of HDX-MS and XL-MS workflows
• Follow specific workflows for sample preparation, including deuterium labeling, protein crosslinking, protein digestion, and sample clean-up
• LC-MS/MS instrument operation and acquisition of HDX-MS data
• Data analysis of HDX-MS and XL-MS data

Generate montly and quarterly reports of HDX-MS and XL-MS data, including visualization tools and protein structure figures

Instrument Management

• Daily operation and routine maintenance of state-of-the-art LC-MS/MS instrumentation
• Operation of instrument software for instrument calibration and data acquisition
• Performance of instrument QA/QC procedures

Maintenance

• Routine laboratory maintenance
• Supply ordering
• Data management
• Record-keeping

Chemical waste disposal

Supervisory Relationships:

This position does not have supervisory responsibility.

Education and Certifications: Profile of an Ideal Candidate

PhD in physical or biological sciences is required.

Qualifications:

• At least five years of post-graduate experience required.
• Laboratory experience in proteomics, biochemistry, or analytical chemistry is required
• Experience in protein/peptide analysis using HPLC - tandem mass spectrometry (LC-MS/MS), and mass spectrometry data analysis is required.

Skills:

• Strong organizational skills
• Strong written and verbal communication skills
• Ability to learn and assist in development of new protocols in an evolving field