Senior Associate Scientist-Molecular and Cellular Biology (Onsite San Diego, CA)

Senior Associate Scientist-Molecular and Cellular Biology (Onsite- San Diego, CA)

(San Diego, CA, 92108) | 2025-08-20 13:34:03

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Job Description

Senior Associate Scientist-Molecular and Cellular Biology (Onsite- San Diego, CA)

We are seeking a Senior Associate Scientist-Molecular and Cellular Biology for a global pharmaceutical client. This role focuses on delivering novel cancer therapeutics by engaging E3 ubiquitin ligases for targeted protein degradation. with broad experience and a strong understanding of small-molecule drug discovery processes. The successful candidate will be developing and implementing robust cellular assays to support the discovery of small molecules as potential clinical candidates; and executing in vitro studies to elucidate the mechanisms of action of novel compounds.

This is a 40-hour per-week, 12-month contract (extensions possible), 100% onsite role in San Diego, CA. Work Schedule- M-F.

This is a W2 role as a Stage 4 Solutions employee. Health benefits and a 401K are offered.

Responsibilities:

Responsibilities include utilizing cellular models to develop and execute cell line construction, gene editing and development of new in vitro assays.

The candidate will actively contribute to discovery project teams, collaborate with scientists across various functional areas, and play a key role in project-based decision-making.

Developing and implementing cell-based and biochemical assays to support the identification, optimization, and characterization of novel chemical matter.

Designing and constructing cell lines and molecular tools for mechanism of action studies (MoA).

Optimizing and assisting with DNA, RNA, and protein purification assays.

Designing and conducting critical experiments by leveraging expertise in cell biology, molecular biology, biochemistry, and assay development to advance mechanism of action (MoA) studies.

Analyzing and presenting data in a clear, organized and timely manner.

Support biology needs for various projects and cross-functional drug discovery teams.

Requirements:

3-4 years of experience with mammalian cell culture techniques and cell-based assays.

Hands-on experience with lentivirus preparation, cell line transfection, CRISPR KO nucleofection, siRNA and shRNA knockdowns, western blot and immunofluorescence analysis.

Proficient in protein and nucleic acid techniques, including Western blotting, SDS-PAGE, qPCR and plasmid prep.

Strong data analysis and management skills.

Ability to use laboratory automation equipment and to develop assays in 96- or 384-well formats.

Strong understanding of in vitro cell-based assays, including but not limited to cell proliferation, cell cycle, and apoptosis assays, as well as MSD, and Incucyte-based growth assays.

Familiarity with the discovery and optimization of novel small molecule entities, including lead identification and an understanding of key aspects of Structure-Activity Relationship (SAR) support using biological assays, is a plus.

Experience with genome-wide screening techniques and genomics, including RNAseq, is a plus

Bachelor's degree in biology or MS.