Postdoctoral Research Associate - Center of Excellence for Pediatric Immuno-Oncology - Fontana Lab

A postdoctoral research associate position is immediately available in the lab of Dr. Pietro Fontana.

Project Description:
Dr. Pietro Fontana , Assistant Member in the Center of Excellence for Pediatric Immuno-Oncology (CEPIO) and Department of Immunology, is seeking passionate and highly motivated candidates for a fully funded postdoctoral fellowship in either St r uctural Biology or Cell Biology . Candidates with specialty in either field are encouraged to apply. Please include a cover letter describing your interest.

The Fontana lab focuses on understanding how immune receptors recognize danger signals and initiate downstream responses, with a particular emphasis on the mechanistic basis of activation and small molecule modulation. We investigate pathways involving cytosolic DNA sensors, inflammasome components, and adaptor proteins such as cGAS , AIM2, and TLRs . Our research integrates cryo-EM, cell biology, and chemical biology to dissect how molecular architecture governs immune function. Recent work includes the discovery and structural characterization of novel dsDNA sensors and the identification of small molecules targeting key innate immune components .

Position Responsibilities:
Option 1: Structural Biology

Express and purify immune signaling proteins and complexes using bacterial, insect, and mammalian systems.

Prepare samples for structural studies by cryo-electron microscopy (cryo-EM) and/or X-ray crystallography.

Perform data processing and model building (e.g., Relion , CryoSPARC , Coot, PyMOL ).

Option 2 : Cell Biology

Design and execute cell-based assays to investigate innate immune signaling pathways, including interferon responses, NfKb pathway and cell death.

Engineer and characterize genetic models, including CRISPR/Cas9 knockouts, and inducible systems in mammalian cells.

Analyze immune pathway activation using molecular biology techniques (qPCR, Western blotting, ELISA, flow cytometry) and microscopy.

Additional training in immunology, structural biology , biochemistry, and cell biology will be provided for highly motivated candidates.

Minimum Education and/or Training:
Successful applicants will hold a Ph.D. in cell biology, structural biology or related discipline. The candidate should demonstrate an academic record of excellence, and a strong interest in the growing field of structural immunology.

Special Skills, Knowledge, and Abilities:
Option 1 : Structural Biology

• Experience with protein expression, purification, and biochemical characterization using bacterial, insect or mammalian cell culture.
  

• Good working knowledge of single particle cryo-EM pipeline and data processing.
  

• Prior knowledge of basic cell culture, and cell-based assays
  

Option 2 : Cell Biology

• Experience with mammalian cell culture, gene editing (CRISPR/Cas9), and generation of stable cell lines (lentivirus).
  

• Strong background in cell signaling, innate immunity, or related areas.
  

• Proficiency in molecular biology and immunoassays (qPCR, Western blotting, ELISA, flow cytometry, reporter assays).
  

• Experience with fluorescence microscopy and imaging-based assays.
  

• E xcellent communication and presentation skills and contribute to preparation of high-quality manuscripts.
  

About the Lab and Institution:
The Fontana Lab fosters a collaborative and dynamic research environment, deeply committed to the professional development of its members. We value ideas and love for science and encourage active participation in growing opportunities, such as grant writing, attendance at immersive workshops, and presenting research at internal and external me e tings .

St. Jude Children's Research Hospital is a world- class research institution focused on groundbreaking pediatric cancer research. With access to state-of-the-art core facilities, cutting-edge technologies, and a vibrant scientific community, we provide an ideal environment for postdoctoral researchers to thrive and make significant contributions to the field of cancer immunotherapy.

Why Join Us?

• State-of-the-Art Resources : Access to institutional core facilities equipped with advanced technologies and led by expert scientists.
  

• Tailored Career Development : Personalized programs designed to enhance professional growth, including grant writing workshops, mentorship opportunities, and more.
  

• Competitive Compensation : Starting salary of $7 2 , 530 per year, plus comprehensive benefits, and relocation assistance .
  

St. Jude is an Equal Opportunity Employer

No Search Firms

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